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Visualizing the DNA repair process by a photolyase at atomic resolution.
- Source :
-
Science (New York, N.Y.) [Science] 2023 Dec; Vol. 382 (6674), pp. eadd7795. Date of Electronic Publication: 2023 Dec 01. - Publication Year :
- 2023
-
Abstract
- Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution.
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 382
- Issue :
- 6674
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 38033054
- Full Text :
- https://doi.org/10.1126/science.add7795