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Characterization and genome analysis of Neobacillus mesonae NS-6, a ureolysis-driven strain inducing calcium carbonate precipitation.
- Source :
-
Frontiers in microbiology [Front Microbiol] 2023 Nov 01; Vol. 14, pp. 1277709. Date of Electronic Publication: 2023 Nov 01 (Print Publication: 2023). - Publication Year :
- 2023
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Abstract
- In this study, a highly promising bacterium was isolated from sandstone oil in the Ordos Basin, named strain NS-6 which exhibited exceptional urease production ability and demonstrated superior efficiency in inducing the deposition of calcium carbonate (CaCO <subscript>3</subscript> ). Through morphological and physiochemical characteristics analysis, as well as 16S rRNA sequencing, strain NS-6 was identified as Neobacillus mesonae . The activity of urease and the formation of CaCO <subscript>3</subscript> increased over time, reaching a maximum of 7.9 mmol/L/min and 184 mg (4.60 mg/mL) respectively at 32 h of incubation. Scanning Electron Microscopy (SEM) revealed CaCO <subscript>3</subscript> crystals ranging in size from 5 to 6 μm, and Energy Dispersive X-ray (EDX) analysis verified the presence of calcium, carbon, and oxygen within the crystals. X-ray Diffraction (XRD) analysis further confirmed the composition of these CaCO <subscript>3</subscript> crystals as calcite and vaterite. Furthermore, the maximum deposition of CaCO <subscript>3</subscript> by strain NS-6 was achieved using response surface methodology (RSM), amounting to 193.8 mg (4.845 mg/mL) when the concentration of calcium ions was 0.5 mmol/L supplemented with 0.9 mmol/L of urea at pH 8.0. Genome-wide analysis revealed that strain NS-6 possesses a chromosome of 5,736,360 base pairs, containing 5,442 predicted genes, including 3,966 predicted functional genes and 1,476 functionally unknown genes. Genes like ure A, ure B, and ure C related to urea catabolism were identified by gene annotation, indicating that strain NS-6 is a typical urease-producing bacterium and possesses a serial of genes involved in metabolic pathways that mediated the deposition of CaCO <subscript>3</subscript> at genetic level.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2023 Xu, Zhang, Ma, Rao and Ma.)
Details
- Language :
- English
- ISSN :
- 1664-302X
- Volume :
- 14
- Database :
- MEDLINE
- Journal :
- Frontiers in microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38029179
- Full Text :
- https://doi.org/10.3389/fmicb.2023.1277709