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circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis.
- Source :
-
Biochemical genetics [Biochem Genet] 2024 Aug; Vol. 62 (4), pp. 2853-2868. Date of Electronic Publication: 2023 Nov 29. - Publication Year :
- 2024
-
Abstract
- Hypopharyngeal squamous cell carcinoma (HSCC) is a malignant tumor of head and neck. It was verified that circ0005027 was downregulated in HSCC tissues. Here, we aimed to investigate the function and specific regulatory mechanism of circ0005027 in HSCC. Ten pairs tissues of HSCC and adjacent para-cancer were collected. Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) measured circ0005027, miR-548c-3p, and Cadherin 1 (CDH1) mRNA expression. CCK-8 analyzed cell proliferation viability. Flow cytometry assay detected cell cycle and apoptosis rate. Clonal formation assay measured the clonal ability. Transwell detected cell invasion ability. Western blot was performed to detect CDH1, LAST1, p-LAST1, MST1, p-MST1, YAP1, p-YAP1, TAZ and p-TAZ protein level. Dual-luciferase, RIP and RNA pull-down assay identified the target relationship among circ0005027, miR-548c-3p and CDH1. circ0005027 was decreased in tissues and FaDu cells of HSCC. Overexpression of circ0005027 inhibited cell viability, G1-S transition, clonal formation, and invasion and increased cell apoptosis. circ0005027 acted as a ceRNA and decreased circ0005027 enhanced the malignant process of FaDu cells through sponging miR-548c-3p and inhibiting CDH1 expression. Overexpression of CDH1 activated YAP1/TAZ pathway and inhibited the growth of HSCC in vitro. circ0005027 might act as a potential biomarker for the progression and prognosis prediction in HSCC by regulating miR-548c-3p/CDH1/ YAP1/TAZ signaling pathway.<br /> (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Humans
Gene Expression Regulation, Neoplastic
Squamous Cell Carcinoma of Head and Neck genetics
Squamous Cell Carcinoma of Head and Neck metabolism
Squamous Cell Carcinoma of Head and Neck pathology
Cell Line, Tumor
Cell Proliferation
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Female
Apoptosis
RNA, Competitive Endogenous
MicroRNAs genetics
MicroRNAs metabolism
Cadherins genetics
Cadherins metabolism
Hypopharyngeal Neoplasms genetics
Hypopharyngeal Neoplasms metabolism
Hypopharyngeal Neoplasms pathology
Antigens, CD genetics
Antigens, CD metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4927
- Volume :
- 62
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical genetics
- Publication Type :
- Academic Journal
- Accession number :
- 38019338
- Full Text :
- https://doi.org/10.1007/s10528-023-10570-y