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Cell wall-localized Bt protein endows rice high resistance to Lepidoptera pests.
- Source :
-
Pest management science [Pest Manag Sci] 2024 Apr; Vol. 80 (4), pp. 1728-1739. Date of Electronic Publication: 2023 Dec 14. - Publication Year :
- 2024
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Abstract
- Background: The commercialized Bt (Bacillus thuringiensis) crops accumulate Bt protein within cells, but the intracellular interactions of foreign protein with endogenous protein inevitably result in large or small unintended effects. In this study, the Bt gene Cry1Ca was linked with the sequences of extracellular secretion signal peptide and carbohydrate binding module 11 to constitute a fusion gene SP-Cry1Ca-CBM11, and the fusion gene driven by constitutive promoters was used for secreting and anchoring onto the cell wall to minimize unintended effects.<br />Results: The transient expression in tobacco leaves demonstrated that the fusion protein was anchored on cell walls. The Cry1Ca contents of five homozygous rice transformants of single-copy insertion were different and descended in the order leaf > root > stem. The maximum content of Cry1Ca was 17.55 μg g <superscript>-1</superscript> in leaves of transformant 21H037. The bioassay results revealed that the transformants exhibited high resistance to lepidopteran pests. The corrected mortality of pink stem borer (Sesamia inferens) and striped stem borer (Chilo suppressalis) ranged from 96.33% to 100%, and from 83.32% to 100%, respectively, and the corrected mortality of rice leaf roller (Cnaphalocrocis medinalis) was 92.53%. Besides, the agronomic traits of the five transformants were normal and similar to that of the recipient, and the transformants were highly resistant to glyphosate at the germination and seedling stages.<br />Conclusion: The fusion Bt protein was accumulated on cell walls and endowed the rice with high resistance to lepidopteran pests without unintended effects in agronomic traits. © 2023 Society of Chemical Industry.<br /> (© 2023 Society of Chemical Industry.)
- Subjects :
- Animals
Endotoxins pharmacology
Bacillus thuringiensis Toxins metabolism
Bacillus thuringiensis Toxins pharmacology
Bacterial Proteins pharmacology
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Hemolysin Proteins genetics
Hemolysin Proteins pharmacology
Hemolysin Proteins metabolism
Pest Control, Biological methods
Lepidoptera genetics
Oryza genetics
Oryza metabolism
Moths
Bacillus thuringiensis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1526-4998
- Volume :
- 80
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Pest management science
- Publication Type :
- Academic Journal
- Accession number :
- 38009289
- Full Text :
- https://doi.org/10.1002/ps.7901