New insights from poly-lactic acid and ionomer films coupled with recombinant antibodies for processing sexed-sorting bovine sperm.

In this study, the efficacy of ionomers and poly-lactic acid (PLA) as an alternative solid material combined with scFv antibodies specific to bovine Y-sperm (Y-scFv) was studied to create a novel method of sexing technology. The coupling efficiency of Y-scFv to the surface of PLA, Na ⁺ and Zn ²⁺ ionomer film was between 2 and 8 mg/mL. Fourier transform infrared spectra confirm that Y-scFv was bound with a carboxylic acid group in each film. Therefore, Na ⁺ , Zn ²⁺ ionomers and PLA films conjugated with 4 and 8 mg/mL Y-scFv showed the highest concentration of Y-sperm in the eluted fraction. Considering that the elute fraction was enriched Y-sperm fraction, it contained 67.70-77.94 % of the Y-sperm ratio related to the produced supernatant fraction, which contained up to 69.31-76.01 % enriched X-sperm. In addition, the sperm quality after the sexing process was analyzed by CASA and imaging flow cytometry, which showed that each polymer did not have a negative effect on sperm motility and acrosome integrity for X-sperm. The capacity of ionomer and PLA combined with Y-scFv are used for bovine sperm sexing.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Korawan Sringarm reports financial support was provided by Chiang Mai University. Korawan Sringarm has patent #2101007525 pending to Licensee.
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