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BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in Pseudomonas aeruginosa .
- Source :
-
Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2023 Oct 28; Vol. 12 (11). Date of Electronic Publication: 2023 Oct 28. - Publication Year :
- 2023
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Abstract
- Objectives: The BIChromET selective medium for detecting piperacillin-tazobactam (TZP) and cefepime (FEP) resistant Pseudomonas aeruginosa was developed.<br />Methods: The performance of this medium was first evaluated using a collection of 100 P. aeruginosa clinical strains (70 TZP-susceptible, 30 TZP-resistant, 58 FEP-susceptible, and 42 FEP-resistant). Then, we performed clinical validation by testing 173 respiratory clinical samples.<br />Results: The BIChromET medium showed excellent sensitivity (TZP (avg. 96.7%); FEP (avg. 92.7%)) and specificity (TZP (avg. 98.9%); FEP (avg. 98%)) in distinguishing the detection limit ranging from 10 <superscript>4</superscript> to 10 <superscript>8</superscript> CFU/mL. Then, testing the bronchoalveolar lavage (BAL) and tracheobronchial aspirate (TBA) clinical specimens (N = 173) revealed the excellent performance of the medium with P. aeruginosa , showing 100% and 92.6% of categorical agreements with the results obtained via the broth microdilution methods (BMD) for TZP and FEP, respectively.<br />Conclusion: This medium allows for easy and accurate detection of TZP/FEP-resistant isolates regardless of their resistance mechanisms.
Details
- Language :
- English
- ISSN :
- 2079-6382
- Volume :
- 12
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Antibiotics (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 37998775
- Full Text :
- https://doi.org/10.3390/antibiotics12111573