[Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor].

Authors :: Macchia V
Rocino A
Gulletta E
Consiglio E
Varrone S
Source :: Comptes rendus des seances de la Societe de biologie et de ses filiales [C R Seances Soc Biol Fil] 1978; Vol. 172 (5), pp. 860-7.
Publication Year :: 1978
Abstract: Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated.

Subjects :: Enzyme Inhibitors isolation & purification
Guanylate Cyclase antagonists & inhibitors
Bacterial Proteins isolation & purification
Escherichia coli enzymology
Guanylate Cyclase isolation & purification

Language :: French
ISSN :: 0037-9026
Volume :: 172
Issue :: 5
Database :: MEDLINE
Journal :: Comptes rendus des seances de la Societe de biologie et de ses filiales
Publication Type :: Academic Journal
Accession number :: 37997

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