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Circulating tumour cells and PD-L1-positive small extracellular vesicles: the liquid biopsy combination for prognostic information in patients with metastatic non-small cell lung cancer.

Authors :
Eslami-S Z
Cortés-Hernández LE
Sinoquet L
Gauthier L
Vautrot V
Cayrefourcq L
Avoscan L
Jacot W
Pouderoux S
Viala M
Thomas QD
Lamy PJ
Quantin X
Gobbo J
Alix-Panabières C
Source :
British journal of cancer [Br J Cancer] 2024 Jan; Vol. 130 (1), pp. 63-72. Date of Electronic Publication: 2023 Nov 16.
Publication Year :
2024

Abstract

Background: Circulating tumour cells (CTCs), circulating tumour DNA (ctDNA), and extracellular vesicles (EVs) are minimally invasive liquid biopsy biomarkers. This study investigated whether they predict prognosis, alone or in combination, in heterogenous unbiased non-small cell lung cancer (NSCLC) patients.<br />Methods: Plasma samples of 54 advanced NSCLC patients from a prospective clinical trial. CtDNA mutations were identified using the UltraSEEK™ Lung Panel (MassARRAY® technology). PD-L1 expression was assessed in small EVs (sEVs) using an enzyme-linked immunosorbent assay.<br />Results: At least one ctDNA mutation was detected in 37% of patients. Mutations were not correlated with overall survival (OS) (HR = 1.1, 95% CI = 0.55; 1.83, P = 0.980) and progression-free survival (PFS) (HR = 1.00, 95% CI = 0.57-1.76, P = 0.991). High PD-L1 <superscript>+</superscript> sEV concentration was correlated with OS (HR = 1.14, 95% CI = 1.03-1.26, P = 0.016), but not with PFS (HR = 1.08, 95% CI = 0.99-1.18, P = 0.095). The interaction analysis suggested that PD-L1 <superscript>+</superscript> sEV correlation with PFS changed in function of CTC presence/absence (P interaction = 0.036). The combination analysis highlighted worse prognosis for patients with CTCs and high PD-L1 <superscript>+</superscript> sEV concentration (HR = 7.65, 95% CI = 3.11-18.83, P < 0.001). The mutational statuses of ctDNA and tumour tissue were significantly correlated (P = 0.0001).<br />Conclusion: CTCs and high PD-L1 <superscript>+</superscript> sEV concentration correlated with PFS and OS, but not ctDNA mutations. Their combined analysis may help to identify patients with worse OS.<br />Trial Registration: NCT02866149, Registered 01 June 2015, https://clinicaltrials.gov/ct2/show/study/NCT02866149 .<br /> (© 2023. The Author(s).)

Details

Language :
English
ISSN :
1532-1827
Volume :
130
Issue :
1
Database :
MEDLINE
Journal :
British journal of cancer
Publication Type :
Academic Journal
Accession number :
37973956
Full Text :
https://doi.org/10.1038/s41416-023-02491-9