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The Azotobacter vinelandii AlgU regulon during vegetative growth and encysting conditions: A proteomic approach.
- Source :
-
PloS one [PLoS One] 2023 Nov 15; Vol. 18 (11), pp. e0286440. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- In the Pseduomonadacea family, the extracytoplasmic function sigma factor AlgU is crucial to withstand adverse conditions. Azotobacter vinelandii, a closed relative of Pseudomonas aeruginosa, has been a model for cellular differentiation in Gram-negative bacteria since it forms desiccation-resistant cysts. Previous work demonstrated the essential role of AlgU to withstand oxidative stress and on A. vinelandii differentiation, particularly for the positive control of alginate production. In this study, the AlgU regulon was dissected by a proteomic approach under vegetative growing conditions and upon encystment induction. Our results revealed several molecular targets that explained the requirement of this sigma factor during oxidative stress and extended its role in alginate production. Furthermore, we demonstrate that AlgU was necessary to produce alkyl resorcinols, a type of aromatic lipids that conform the cell membrane of the differentiated cell. AlgU was also found to positively regulate stress resistance proteins such as OsmC, LEA-1, or proteins involved in trehalose synthesis. A position-specific scoring-matrix (PSSM) was generated based on the consensus sequence recognized by AlgU in P. aeruginosa, which allowed the identification of direct AlgU targets in the A. vinelandii genome. This work further expands our knowledge about the function of the ECF sigma factor AlgU in A. vinelandii and contributes to explains its key regulatory role under adverse conditions.<br />Competing Interests: The authors have declared that no competing interests exist.<br /> (Copyright: © 2023 Chowdhury-Paul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Subjects :
- Regulon genetics
Proteomics
Heat-Shock Proteins metabolism
Alginates metabolism
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Pseudomonas aeruginosa genetics
Sigma Factor genetics
Sigma Factor metabolism
Azotobacter vinelandii genetics
Azotobacter vinelandii metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 18
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 37967103
- Full Text :
- https://doi.org/10.1371/journal.pone.0286440