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Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly.

Authors :
Bañón A
Alsina B
Source :
Development (Cambridge, England) [Development] 2023 Nov 01; Vol. 150 (21). Date of Electronic Publication: 2023 Nov 08.
Publication Year :
2023

Abstract

Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show that otic NBs delaminate out of the otic epithelium in an epithelial-mesenchymal transition-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NBs require RhoGTPases in order to perform active migration. Furthermore, tracking of recently delaminated NBs revealed their directed migration and coalescence around a small population of pioneer SAG neurons. These pioneer SAG neurons, not from otic placode origin, populate the coalescence region before otic neurogenesis begins and their ablation disrupts delaminated NB migratory pathways, consequentially affecting SAG shape. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.<br />Competing Interests: Competing interests The authors declare no competing or financial interests.<br /> (© 2023. Published by The Company of Biologists Ltd.)

Details

Language :
English
ISSN :
1477-9129
Volume :
150
Issue :
21
Database :
MEDLINE
Journal :
Development (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
37938828
Full Text :
https://doi.org/10.1242/dev.201824