Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections.

Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as K. kingae and N. gonorrhoeae. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.
Competing Interests: Declaration of Competing Interest Llanos Salar-Vidal has received speaker honoraria from Werfen. Patricio Favier has received consultant honoraria from Biomérieux Argentina. Jaime Esteban has received speaker honoraria from Biomérieux and Heraeus, and consultant honoraria from Biomérieux. Catarina Chaves, Ileana T. Dianzo-Delgado, Salvador Giner-Almaraz, María José Gómez-Gómez, Guillermo Martín-Gutiérrez, Isabel Pereira, Ana Rodríguez-Fernández, Patricia Ruiz-Garbajosa, and Carlos Salas-Venero declare they have no financial interests.
(Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)