A strategy for orthogonal deubiquitination using a bump-and-hole approach.

Authors :: Suzuki T
Utsugi Y
Yamanaka S
Takahashi H
Sato Y
Sawasaki T
Miyamae Y
Source :: RSC chemical biology [RSC Chem Biol] 2023 Oct 02; Vol. 4 (11), pp. 879-883. Date of Electronic Publication: 2023 Oct 02 (Print Publication: 2023).
Publication Year :: 2023
Abstract: We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in living cells.<br />Competing Interests: There are no conflicts to declare.<br /> (This journal is © The Royal Society of Chemistry.)