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Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donors' Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia.
Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donors' Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia.
- Source :
-
The American journal of psychiatry [Am J Psychiatry] 2024 Jun 01; Vol. 181 (6), pp. 493-511. Date of Electronic Publication: 2023 Nov 02. - Publication Year :
- 2024
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Abstract
- Objective: Schizophrenia is a brain disorder that originates during neurodevelopment and has complex genetic and environmental etiologies. Despite decades of clinical evidence of altered striatal function in affected patients, studies examining its cellular and molecular mechanisms in humans are limited. To explore neurodevelopmental alterations in the striatum associated with schizophrenia, the authors established a method for the differentiation of induced pluripotent stem cells (iPSCs) into ventral forebrain organoids (VFOs).<br />Methods: VFOs were generated from postmortem dural fibroblast-derived iPSCs of four individuals with schizophrenia and four neurotypical control individuals for whom postmortem caudate genotypes and transcriptomic data were profiled in the BrainSeq neurogenomics consortium. Individuals were selected such that the two groups had nonoverlapping schizophrenia polygenic risk scores (PRSs).<br />Results: Single-cell RNA sequencing analyses of VFOs revealed differences in developmental trajectory between schizophrenia and control individuals in which inhibitory neuronal cells from the patients exhibited accelerated maturation. Furthermore, upregulated genes in inhibitory neurons in schizophrenia VFOs showed a significant overlap with upregulated genes in postmortem caudate tissue of individuals with schizophrenia compared with control individuals, including the donors of the iPSC cohort.<br />Conclusions: The findings suggest that striatal neurons derived from high-PRS individuals with schizophrenia carry abnormalities that originated during early brain development and that the VFO model can recapitulate disease-relevant cell type-specific neurodevelopmental phenotypes in a dish.
- Subjects :
- Humans
Corpus Striatum pathology
Corpus Striatum metabolism
Male
Female
Cell Differentiation genetics
Transcriptome
Adult
Neurons pathology
Neurons metabolism
Single-Cell Analysis
Schizophrenia genetics
Schizophrenia pathology
Schizophrenia metabolism
Organoids pathology
Organoids metabolism
Induced Pluripotent Stem Cells metabolism
Basal Forebrain pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-7228
- Volume :
- 181
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The American journal of psychiatry
- Publication Type :
- Academic Journal
- Accession number :
- 37915216
- Full Text :
- https://doi.org/10.1176/appi.ajp.20220723