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Identification and expression profiles of olfactory-related genes based on transcriptome analysis in Plodia interpunctella (Lepidoptera: Pyralidae).
- Source :
-
Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2023 Dec; Vol. 114 (4), pp. e22061. Date of Electronic Publication: 2023 Oct 31. - Publication Year :
- 2023
-
Abstract
- The sophisticated olfactory system of insects is plays a critical role in detecting chemical signals and guiding insect behaviors, such as selecting mates, finding hosts, evading predators, and discovering oviposition sites. Therefore, exploring and clarifying the molecular processes of this system is crucial for developing new insecticides or efficient pest control methods. Plodia interpunctella (Hübner) is a disruptive insect pest damaging the stored grains over the world. However, the olfactory processes of P. interpunctella remain unclear. Herein, we employed a transcriptome analysis to identify olfactory and differentially expressed genes to characterize their expression patterns in different developmental stages and antennal tissue. Subsequently, a total of 172 potential olfactory-related genes included 42 odorant-binding proteins, 12 chemosensory proteins, 51 odorant receptors, 13 gustatory receptors, three sensory neuron membrane proteins, and 51 ionotropic receptors. Furthermore, phylogenetic analysis and BLASTx best-hit analyses showed that these olfactory genes were closely linked with those identified in other lepidopterans. Transcriptome analysis revealed 49 differentially expressed olfactory-related genes, and a semiquantitative reverse transcription polymerase chain reaction showed that 11 olfactory genes were particularly expressed in the legs and wings of female P. interpunctella. Meanwhile, PintOBP29 was notably expressed in female antennae and legs. Genes with high expression levels in the abdomen showed high expression in the legs, but low expression in the antennae. Our findings provide the candidate genetic factors for analysis of the olfactory processes in P. interpunctella.<br /> (© 2023 Wiley Periodicals LLC.)
- Subjects :
- Female
Animals
Transcriptome
Phylogeny
Gene Expression Profiling
Receptors, Cell Surface metabolism
Arthropod Antennae metabolism
Insect Proteins genetics
Insect Proteins metabolism
Lepidoptera genetics
Lepidoptera metabolism
Moths genetics
Moths metabolism
Receptors, Odorant genetics
Receptors, Odorant metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6327
- Volume :
- 114
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of insect biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 37905450
- Full Text :
- https://doi.org/10.1002/arch.22061