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Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy.
- Source :
-
Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2023 Oct 08; Vol. 9 (10). Date of Electronic Publication: 2023 Oct 08. - Publication Year :
- 2023
-
Abstract
- (1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto , M. pinghaense , M. robertsii and M. brunneum . Unambiguous species delineation within this "PARB clade" that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
Details
- Language :
- English
- ISSN :
- 2309-608X
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of fungi (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 37888252
- Full Text :
- https://doi.org/10.3390/jof9100996