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Systematic metabolic engineering of Yarrowia lipolytica for the enhanced production of erythritol.
- Source :
-
Bioresource technology [Bioresour Technol] 2024 Jan; Vol. 391 (Pt A), pp. 129918. Date of Electronic Publication: 2023 Oct 24. - Publication Year :
- 2024
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Abstract
- In recent times, there has been a growing interest in exploring microbial strains that exhibit enhanced erythritol productivity. Nonetheless, the lack of advanced synthetic biology tools has limited rapid strain development. In this study, the CRISPR/Cas9 system was employed to genetically modify Yarrowia lipolytica at the chromosomal level, which could improve the production of erythritol while saving the time required to markers recovery, and realizing the rapid construction of high-erythritol strains. Firstly, the basic strain E004 was generated by increasing the efficiency of homologous recombination and regulating the erythritol degradation pathway. Secondly, eleven key gene targets and a strong promoter 8UAS1B <subscript>XPR2</subscript> -P <subscript>TEFin</subscript> was obtained by target screening and promoter engineering. Finally, based on modular pathway engineering and morphological engineering, the high production of erythritol was achieved successfully. The best-engineered strain E326 produced 256 g/L erythritol in a 5-L bioreactor, which is the highest production level reported so far in Y. lipolytica.<br />Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Zhiming Rao reports financial support was provided by National Key Research and Development Program of China. Zhiming Rao reports financial support was provided by National Natural Science Foundation of China. Xuewei Pan reports financial support was provided by Natural Science Foundation of Jiangsu Province. Zhiming Rao reports financial support was provided by Program of Introducing Talents of Discipline to Universities. Zhiming Rao reports financial support was provided by National First-class Discipline Program of Light Industry Technology and Engineering. Shuling Yang reports financial support was provided by Postgraduate Research & Practice Innovation Program of Jiangsu Provence.<br /> (Copyright © 2023 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 391
- Issue :
- Pt A
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 37884093
- Full Text :
- https://doi.org/10.1016/j.biortech.2023.129918