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Expanding diversity of bunyaviruses identified in mosquitoes.

Authors :
Orba Y
Abu YE
Chambaro HM
Lundu T
Muleya W
Eshita Y
Qiu Y
Harima H
Kajihara M
Mori-Kajihara A
Matsuno K
Sasaki M
Hall WW
Hang'ombe BM
Sawa H
Source :
Scientific reports [Sci Rep] 2023 Oct 24; Vol. 13 (1), pp. 18165. Date of Electronic Publication: 2023 Oct 24.
Publication Year :
2023

Abstract

Mosquitoes interact with various organisms in the environment, and female mosquitoes in particular serve as vectors that directly transmit a number of microorganisms to humans and animals by blood-sucking. Comprehensive analysis of mosquito-borne viruses has led to the understanding of the existence of diverse viral species and to the identification of zoonotic arboviruses responsible for significant outbreaks and epidemics. In the present study on mosquito-borne bunyaviruses we employed a broad-spectrum RT-PCR approach and identified eighteen different additional species in the Phenuiviridae family and also a number of related but unclassified bunyaviruses in mosquitoes collected in Zambia. The entire RNA genome segments of the newly identified viruses were further analyzed by RNA sequencing with a ribonuclease R (RNase R) treatment to reduce host-derived RNAs and enrich viral RNAs, taking advantage of the dsRNA panhandle structure of the bunyavirus genome. All three or four genome segments were identified in eight bunyavirus species. Furthermore, L segments of three different novel viruses related to the Leishbunyaviridae were found in mosquitoes together with genes from the suspected host, the Crithidia parasite. In summary, our virus detection approach using a combination of broad-spectrum RT-PCR and RNA sequencing analysis with a simple virus enrichment method allowed the discovery of novel bunyaviruses. The diversity of bunyaviruses is still expanding and studies on this will allow a better understanding of the ecology of hematophagous mosquitoes.<br /> (© 2023. Springer Nature Limited.)

Details

Language :
English
ISSN :
2045-2322
Volume :
13
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
37875565
Full Text :
https://doi.org/10.1038/s41598-023-45443-2