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Turn-on Rhodamine Glycoconjugates Enable Real-Time GLUT Activity Monitoring in Live Cells and In Vivo.
- Source :
-
Chemical & biomedical imaging [Chem Biomed Imaging] 2023 Oct 23; Vol. 1 (7), pp. 637-647. Date of Electronic Publication: 2023 Sep 01. - Publication Year :
- 2023
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Abstract
- The direct relationship between facilitative glucose transporters (GLUTs) and metabolic diseases opens new avenues for sensing metabolic deregulations and drives the development of molecular probes for GLUT-targeted detection of metabolic diseases. Radiotracer-based molecular imaging probes have been effectively utilized in reporting alterations in sugar uptake as an indication of metabolic deregulations, cancer development, or inflammation. Progress in developing fluorophore-based tools facilitated GLUT-specific analyses using more accessible fluorescence-based instrumentation. However, restrictions on the emission range of fluorophores and the requirement for substantial post-treatments to reduce background fluorescence have brought to light the critical directions for improvement of the technology for broader use in screening applications. Here we present turn-on GLUT activity reporters activated upon cells' internalization. We demonstrate a specific delivery of a sizable rhodamine B fluorophore through GLUT5 and showcase a stringent requirement in conjugate structure for maintaining a GLUT-specific uptake. With the turn-on GLUT probes, we demonstrate the feasibility of high-throughput fluorescence microscopy and flow cytometry-based GLUT activity screening in live cells and the probes' applicability for assessing sugar uptake alterations in vivo .<br />Competing Interests: The authors declare no competing financial interest.
Details
- Language :
- English
- ISSN :
- 2832-3637
- Volume :
- 1
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Chemical & biomedical imaging
- Publication Type :
- Academic Journal
- Accession number :
- 37873027
- Full Text :
- https://doi.org/10.1021/cbmi.3c00063