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Proteomic Serum Profiling of Holstein Friesian Cows with Different Pathological Forms of Bovine Paratuberculosis Reveals Changes in the Acute-Phase Response and Lipid Metabolism.
- Source :
-
Journal of proteome research [J Proteome Res] 2024 Aug 02; Vol. 23 (8), pp. 2762-2779. Date of Electronic Publication: 2023 Oct 20. - Publication Year :
- 2024
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Abstract
- The lack of sensitive diagnostic methods to detect Mycobacterium avium subsp. paratuberculosis (Map) subclinical infections has hindered the control of paratuberculosis (PTB). The serum proteomic profiles of naturally infected cows presenting focal and diffuse pathological forms of PTB and negative controls ( n = 4 per group) were analyzed using TMT-6plex quantitative proteomics. Focal and diffuse are the most frequent pathological forms in subclinical and clinical stages of PTB, respectively. One (focal versus (vs.) control), eight (diffuse vs. control), and four (focal vs. diffuse) differentially abundant (DA) proteins ( q -value < 0.05) were identified. Ingenuity pathway analysis of the DA proteins revealed changes in the acute-phase response and lipid metabolism. Six candidate biomarkers were selected for further validation by specific ELISA using serum from animals with focal, multifocal, and diffuse PTB-associated lesions ( n = 108) and controls ( n = 56). Overall, the trends of the serum expression levels of the selected proteins were consistent with the proteomic results. Alpha-1-acid glycoprotein (ORM1)-based ELISA, insulin-like growth factor-binding protein 2 (IGFBP2)-based ELISA, and the anti-Map ELISA had the best diagnostic performance for detection of animals with focal, multifocal, and diffuse lesions, respectively. Our findings identify potential biomarkers that improve diagnostic sensitivity of PTB and help to elucidate the mechanisms involved in PTB pathogenesis.
- Subjects :
- Animals
Cattle
Acute-Phase Reaction blood
Female
Mycobacterium avium subsp. paratuberculosis pathogenicity
Enzyme-Linked Immunosorbent Assay
Proteome metabolism
Paratuberculosis blood
Paratuberculosis microbiology
Paratuberculosis diagnosis
Lipid Metabolism
Proteomics methods
Cattle Diseases blood
Cattle Diseases microbiology
Cattle Diseases diagnosis
Biomarkers blood
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 37863471
- Full Text :
- https://doi.org/10.1021/acs.jproteome.3c00244