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Evaluation of packaging capacity at the genomic 2C/3A junction region in Porcine enterovirus G.

Authors :
Huang S
Mi X
Ren T
Hong D
Qin Q
Long M
Qin Y
Chen Y
Wei Z
Huang W
Ouyang K
Source :
Virology [Virology] 2023 Nov; Vol. 588, pp. 109899. Date of Electronic Publication: 2023 Oct 13.
Publication Year :
2023

Abstract

Porcine enterovirus G (EV-G) is endogenous to most pig farming countries worldwide. Reports that a papain-like protease (PLP) gene has been naturally inserted into the 2C/3A junction region of the EV-G genome, has increased the potential public health threats from this virus. We constructed a full-length infectious cDNA clone of EV-G, CH/17GXQZ/2017, in order to determine the packaging capacity at the 2C/3A insertion site. Subsequently, recombinants viruses containing the coding tags, GFP, iLOV and His at the 2C/3A junction region, were synthesized. The infectious virus was successfully rescued only with the insertion of the His-tag, which displayed similar virological and molecular properties to its parental strain. This study determined the packaging capacity of the 2C/3A insertion site, and it provides a practical tool for studying the functions and pathogenic mechanisms of EV-G in pigs.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-0341
Volume :
588
Database :
MEDLINE
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
37862828
Full Text :
https://doi.org/10.1016/j.virol.2023.109899