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Matrix metalloproteinase 2 degrades collagen I to regulate ovarian development by association with an insulin-like peptide.
- Source :
-
Insect science [Insect Sci] 2024 Aug; Vol. 31 (4), pp. 1090-1106. Date of Electronic Publication: 2023 Oct 17. - Publication Year :
- 2024
-
Abstract
- The ovary generally undergoes tissue remodeling during larval to pupal transition, which includes membrane degeneration and ovariole growth. At the same time, the hormones produced by insects significantly change during metamorphosis. However, the regulatory mechanism for ovarian development and hormones is not fully understood in insects. Herein, we found that matrix metalloproteinase 2 (MMP2) was highly expressed in the ovarian capsules and ovarioles, and the development was abnormal after knocking out MMP2 in Bombyx mori. The process of abnormal degradation of collagen I due to MMP2 deletion, which resulted in abnormal development of ovarioles and eggs, was analyzed in detail. The proteomics of ovaries in the MMP2-knock out and wild type strains showed a critically significant difference in the expression of a protein, insulin-like peptide (ILP). Additional analysis revealed significant alteration of ILP during ovarian development, and abnormal expression of ILP significantly affected ovarian development in vivo and MMP2 expression in vitro and in vivo. These results showed that MMP2 regulation of ovarian tissue remodeling is closely related to ILP expression. Our study provides new insights into the regulatory mechanism of MMP2 and ovarian development in B. mori.<br /> (© 2023 Institute of Zoology, Chinese Academy of Sciences.)
- Subjects :
- Animals
Female
Collagen Type I metabolism
Collagen Type I genetics
Insect Proteins metabolism
Insect Proteins genetics
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 2 genetics
Bombyx growth & development
Bombyx genetics
Bombyx metabolism
Ovary growth & development
Ovary metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1744-7917
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Insect science
- Publication Type :
- Academic Journal
- Accession number :
- 37846892
- Full Text :
- https://doi.org/10.1111/1744-7917.13284