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A Pseudomonas aeruginosa-Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target mRNAs.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2721, pp. 69-83. - Publication Year :
- 2024
-
Abstract
- Bacterial small RNAs have emerged as modulators that play key roles in regulatory networks related to viability, environmental adaptation, and pathogenesis. Small RNAs can modulate gene expression by base-pairing to target mRNAs, influencing their translation and/or stability. A superfolder GFP reporter system, previously developed for Escherichia coli and Salmonella enterica, was adapted to Pseudomonas aeruginosa and used to validate novel mRNA targets in studies of small RNA-mediated regulatory mechanisms.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Bacterial genetics
RNA, Bacterial metabolism
Escherichia coli genetics
Escherichia coli metabolism
Gene Expression Regulation, Bacterial
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa metabolism
RNA, Small Untranslated genetics
RNA, Small Untranslated metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2721
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 37819516
- Full Text :
- https://doi.org/10.1007/978-1-0716-3473-8_6