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Recyclable CRISPR/Cas9-mediated gene disruption and deletions in Histoplasma .
- Source :
-
MSphere [mSphere] 2023 Dec 20; Vol. 8 (6), pp. e0037023. Date of Electronic Publication: 2023 Oct 11. - Publication Year :
- 2023
-
Abstract
- Importance: Histoplasma is a primary fungal pathogen with the ability to infect otherwise healthy mammalian hosts, causing systemic and sometimes life-threatening disease. Thus far, molecular genetic manipulation of this organism has utilized RNA interference, random insertional mutagenesis, and a homologous recombination protocol that is highly variable and often inefficient. Targeted gene manipulations have been challenging due to poor rates of homologous recombination events in Histoplasma . Interrogation of the virulence strategies of this organism would be highly accelerated by a means of efficiently generating targeted mutations. We have developed a recyclable CRISPR/Cas9 system that can be used to introduce gene disruptions in Histoplasma with high efficiency, thereby allowing disruption of multiple genes.<br />Competing Interests: The authors declare no conflict of interest.
Details
- Language :
- English
- ISSN :
- 2379-5042
- Volume :
- 8
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- MSphere
- Publication Type :
- Academic Journal
- Accession number :
- 37819140
- Full Text :
- https://doi.org/10.1128/msphere.00370-23