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Palmitoylation of the hemagglutinin of influenza B virus by ER-localized DHHC enzymes 1, 2, 4, and 6 is required for efficient virus replication.
- Source :
-
Journal of virology [J Virol] 2023 Oct 31; Vol. 97 (10), pp. e0124523. Date of Electronic Publication: 2023 Oct 04. - Publication Year :
- 2023
-
Abstract
- Importance: Influenza viruses are a public health concern since they cause seasonal outbreaks and occasionally pandemics. Our study investigates the importance of a protein modification called "palmitoylation" in the replication of influenza B virus. Palmitoylation involves attaching fatty acids to the viral protein hemagglutinin and has previously been studied for influenza A virus. We found that this modification is important for the influenza B virus to replicate, as mutating the sites where palmitate is attached prevented the virus from generating viable particles. Our experiments also showed that this modification occurs in the endoplasmic reticulum. We identified the specific enzymes responsible for this modification, which are different from those involved in palmitoylation of HA of influenza A virus. Overall, our research illuminates the similarities and differences in fatty acid attachment to HA of influenza A and B viruses and identifies the responsible enzymes, which might be promising targets for anti-viral therapy.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Humans
Influenza A virus chemistry
Influenza A virus metabolism
Influenza, Human drug therapy
Influenza, Human virology
Mutation
Acyltransferases metabolism
Endoplasmic Reticulum enzymology
Endoplasmic Reticulum virology
Hemagglutinin Glycoproteins, Influenza Virus chemistry
Hemagglutinin Glycoproteins, Influenza Virus genetics
Hemagglutinin Glycoproteins, Influenza Virus metabolism
Influenza B virus chemistry
Influenza B virus growth & development
Influenza B virus metabolism
Lipoylation genetics
Palmitic Acid metabolism
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 97
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 37792001
- Full Text :
- https://doi.org/10.1128/jvi.01245-23