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Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex.
- Source :
-
Viruses [Viruses] 2023 Sep 05; Vol. 15 (9). Date of Electronic Publication: 2023 Sep 05. - Publication Year :
- 2023
-
Abstract
- Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in the canine population and is caused by a wide array of viruses and bacterial pathogens with coinfections being common. Since its recognition in late 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been reported to cause respiratory disease in dogs. Therefore, the rapid detection and differentiation of SARS-CoV-2 from other common viral and bacterial agents is critical from a public health standpoint. Here, we developed and validated a panel of four one-step multiplex qPCR/RT-qPCR assays for the detection and identification of twelve pathogens associated with CIRDC (canine adenovirus-2, canine distemper virus, canine herpesvirus-1, canine influenza A virus, canine parainfluenza virus, canine pneumovirus, canine respiratory coronavirus, SARS-CoV-2, Bordetella bronchiseptica , Streptococcus equi subsp. zooepidemicus , Mycoplasma cynos, and M. canis ), as well as the identification of three main CIV subtypes (i.e., H3N2, H3N8, and H1N1). All developed assays demonstrated high specificity and analytical sensitivity. This panel was used to test clinical specimens ( n = 76) from CIRDC-suspected dogs. M. canis , M. cynos , and CRCoV were the most frequently identified pathogens (30.3%, 25.0%, and 19.7% of samples, respectively). The newly emerging pathogens CPnV and SARS-CoV-2 were detected in 5.3% of samples and coinfections were identified in 30.3%. This new multiplex qPCR/RT-qPCR panel is the most comprehensive panel developed thus far for identifying CIRDC pathogens, along with SARS-CoV-2.
- Subjects :
- Dogs
Animals
SARS-CoV-2 genetics
Influenza A Virus, H3N2 Subtype
Coinfection diagnosis
Coinfection veterinary
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H3N8 Subtype
COVID-19 diagnosis
COVID-19 veterinary
Respiratory Tract Infections diagnosis
Respiratory Tract Infections veterinary
Canidae
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 15
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 37766287
- Full Text :
- https://doi.org/10.3390/v15091881