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In Vivo Intra-Uterine Delivery of TAT-Fused Cre Recombinase and CRISPR/Cas9 Editing System in Mice Unveil Histopathology of Pten/p53-Deficient Endometrial Cancers.
- Source :
-
Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2023 Nov; Vol. 10 (32), pp. e2303134. Date of Electronic Publication: 2023 Sep 25. - Publication Year :
- 2023
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Abstract
- Phosphatase and TENsin homolog (Pten) and p53 are two of the most frequently mutated tumor suppressor genes in endometrial cancer. However, the functional consequences and histopathological manifestation of concomitant p53 and Pten loss of function alterations in the development of endometrial cancer is still controversial. Here, it is demonstrated that simultaneous Pten and p53 deletion is sufficient to cause epithelial to mesenchymal transition phenotype in endometrial organoids. By a novel intravaginal delivery method using HIV1 trans-activator of transcription cell penetrating peptide fused with a Cre recombinase protein (TAT-Cre), local ablation of both p53 and Pten is achieved specifically in the uterus. These mice developed high-grade endometrial carcinomas and a high percentage of uterine carcinosarcomas resembling those found in humans. To further demonstrate that carcinosarcomas arise from epithelium, double Pten/p53 deficient epithelial cells are mixed with wild type stromal and myometrial cells and subcutaneously transplanted to Scid mice. All xenotransplants resulted in the development of uterine carcinosarcomas displaying high nuclear pleomorphism and metastatic potential. Accordingly, in vivo CRISPR/Cas9 disruption of Pten and p53 also triggered the development of metastatic carcinosarcomas. The results unfadingly demonstrate that simultaneous deletion of p53 and Pten in endometrial epithelial cells is enough to trigger epithelial to mesenchymal transition that is consistently translated to the formation of uterine carcinosarcomas in vivo.<br /> (© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)
- Subjects :
- Humans
Female
Mice
Animals
Tumor Suppressor Protein p53 genetics
Tumor Suppressor Protein p53 metabolism
Epithelial-Mesenchymal Transition
CRISPR-Cas Systems genetics
Endometrial Neoplasms genetics
Endometrial Neoplasms metabolism
Endometrial Neoplasms pathology
Uterine Neoplasms genetics
Uterine Neoplasms pathology
Carcinosarcoma genetics
Carcinosarcoma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2198-3844
- Volume :
- 10
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Advanced science (Weinheim, Baden-Wurttemberg, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 37749866
- Full Text :
- https://doi.org/10.1002/advs.202303134