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Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay.
- Source :
-
Avian pathology : journal of the W.V.P.A [Avian Pathol] 2023 Dec; Vol. 52 (6), pp. 438-445. Date of Electronic Publication: 2023 Oct 11. - Publication Year :
- 2023
-
Abstract
- The widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achieved. The detection limit of the target gene with this approach was only 10 <superscript>1</superscript> copies/μl, making it very sensitive and specific. Clinical samples fared well when tested with the Cas13a detection method. For identifying FAdV-4, this novel detection approach was found to be sensitive, specific, and effective. RESEARCH HIGHLIGHTS First study using the CRISPR/Cas13a-based lateral flow detection assay for FAdV-4 detection.The results can be observed by the naked eye.The developed assay could provide an alternative tool for detection of FAdV-4 with minimal equipment.
Details
- Language :
- English
- ISSN :
- 1465-3338
- Volume :
- 52
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Avian pathology : journal of the W.V.P.A
- Publication Type :
- Academic Journal
- Accession number :
- 37746729
- Full Text :
- https://doi.org/10.1080/03079457.2023.2254253