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Synaptotagmin 1-triggered lipid signaling facilitates coupling of exo- and endocytosis.

Authors :
Bolz S
Kaempf N
Puchkov D
Krauss M
Russo G
Soykan T
Schmied C
Lehmann M
Müller R
Schultz C
Perrais D
Maritzen T
Haucke V
Source :
Neuron [Neuron] 2023 Dec 06; Vol. 111 (23), pp. 3765-3774.e7. Date of Electronic Publication: 2023 Sep 21.
Publication Year :
2023

Abstract

Exocytosis and endocytosis are essential physiological processes and are of prime importance for brain function. Neurotransmission depends on the Ca <superscript>2+</superscript> -triggered exocytosis of synaptic vesicles (SVs). In neurons, exocytosis is spatiotemporally coupled to the retrieval of an equal amount of membrane and SV proteins by compensatory endocytosis. How exocytosis and endocytosis are balanced to maintain presynaptic membrane homeostasis and, thereby, sustain brain function is essentially unknown. We combine mouse genetics with optical imaging to show that the SV calcium sensor Synaptotagmin 1 couples exocytic SV fusion to the endocytic retrieval of SV membranes by promoting the local activity-dependent formation of the signaling lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P <subscript>2</subscript> ) at presynaptic sites. Interference with these mechanisms impairs PI(4,5)P <subscript>2</subscript> -triggered SV membrane retrieval but not exocytic SV fusion. Our findings demonstrate that the coupling of SV exocytosis and endocytosis involves local Synaptotagmin 1-induced lipid signaling to maintain presynaptic membrane homeostasis in central nervous system neurons.<br />Competing Interests: Declaration of interests The authors declare no competing financial interests.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1097-4199
Volume :
111
Issue :
23
Database :
MEDLINE
Journal :
Neuron
Publication Type :
Academic Journal
Accession number :
37738980
Full Text :
https://doi.org/10.1016/j.neuron.2023.08.016