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Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans.
- Source :
-
STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102576. Date of Electronic Publication: 2023 Sep 20. - Publication Year :
- 2023
-
Abstract
- The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022). <superscript>1</superscript> .<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 4
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 37733596
- Full Text :
- https://doi.org/10.1016/j.xpro.2023.102576