Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets.

The field of stem cell-based embryo-like models is rapidly evolving, providing in vitro models of in utero stages of mammalian development. Here, we detail steps to first establish adherent spheroids composed of three cell types from mouse embryonic stem cells solely treated with a chemical inhibitor of SUMOylation. We then describe procedures for generating highly reproducible gastruloids from these dissociated spheroid cells, as well as embryo-like structures comprising anterior neural and trunk somite-like regions using an optimized microfluidics platform. For complete details on the use and execution of this protocol, please refer to Cossec et al. (2023). ¹ .
Competing Interests: Declaration of interests Authors are designated as inventors of the patent application WO/2023/002057 covering aspects of the in vitro generation of organized 3D cell structures and the microfluidic chip described in the article.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)