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[Effect of gut microbiota homeostasis on hematopoiesis in a neonatal rat model of necrotizing enterocolitis].
- Source :
-
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics [Zhongguo Dang Dai Er Ke Za Zhi] 2023 Aug 15; Vol. 25 (8), pp. 855-863. - Publication Year :
- 2023
-
Abstract
- Objectives: To study the effect of gut microbiota on hematopoiesis in a neonatal rat model of necrotizing enterocolitis (NEC).<br />Methods: Neonatal Sprague-Dawley rats were randomly divided into a control group and a model group (NEC group), with 6 rats in each group. Formula milk combined with hypoxia and cold stimulation was used to establish a neonatal rat model of NEC. Hematoxylin and eosin staining was used to observe the pathological changes of intestinal tissue and hematopoiesis-related organs. Routine blood tests were conducted for each group. Immunohistochemistry was used to observe the changes in specific cells in hematopoiesis-related organs. Flow cytometry was used to measure the changes in specific cells in bone marrow. 16S rDNA sequencing was used to observe the composition and abundance of gut microbiota.<br />Results: Compared with the control group, the NEC group had intestinal congestion and necrosis, damage, atrophy, and shedding of intestinal villi, and a significant increase in NEC histological score. Compared with the control group, the NEC group had significantly lower numbers of peripheral blood leukocytes and lymphocytes ( P <0.05), nucleated cells in the spleen, thymus, and bone marrow, and small cell aggregates with basophilic nuclei in the liver ( P <0.05). The NEC group had significant reductions in CD71 <superscript>+</superscript> erythroid progenitor cells in the liver, CD45 <superscript>+</superscript> lymphocytes in the spleen and bone marrow, CD3 <superscript>+</superscript> T lymphocytes in thymus, and the proportion of CD45 <superscript>+</superscript> CD3 <superscript>-</superscript> CD43 <superscript>+</superscript> SSC <superscript>hi</superscript> neutrophils in bone marrow ( P <0.05). There was a significant difference in the composition of gut microbiota between the NEC and control groups, and the NEC group had a significant reduction in the abundance of Ligilactobacillus and a significant increase in the abundance of Escherichia - Shigella ( P <0.05), which replaced Ligilactobacillus and became the dominant flora.<br />Conclusions: Multi-lineage hematopoietic disorder may be observed in a neonatal rat model of NEC, which may be associated with gut microbiota dysbiosis and abnormal multiplication of the pathogenic bacteria Escherichia - Shigella .
Details
- Language :
- Chinese
- ISSN :
- 1008-8830
- Volume :
- 25
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 37668035
- Full Text :
- https://doi.org/10.7499/j.issn.1008-8830.2301082