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The Role of Mitochondrial Dysfunction in Cytotoxic Effects of Solanum nigrum Water Extract on MCF-7 and MDA-MB-231 Breast Cancer Cells.

Authors :
Khan HA
Alghamdi AA
Prasad NR
Alrokayan SH
Almansour BS
Hatamilah AAK
Source :
Frontiers in bioscience (Landmark edition) [Front Biosci (Landmark Ed)] 2023 Aug 28; Vol. 28 (8), pp. 180.
Publication Year :
2023

Abstract

Background: Recent studies suggest that numerous naturally occurring agents have the potential to kill cancer cells via mitochondrial dysfunction. Solanum nigrum is a herb widely used in alternative medical systems. This study aimed to investigate the cytotoxic effect of Solanum nigrum water extract (SNWE) against Michigan Cancer Foundation-7 (MCF-7) and MD Anderson-Metastatic Breast Cancer-231 (MDA-MB-231) cells.<br />Methods: We used an MTT reduction assay for cytotoxicity analysis. To explore the mode of action, the cellular adenosine triphosphate (ATP) levels and mitochondrial membrane potential were analyzed using a colorimetric ATP assay and Rhodamine-123 fluorescent staining, respectively, during SNWE treatment for 72 h.<br />Results: The cytotoxic effect was significant in both cell lines, with IC50 values of 4.26 µg/mL and 5.30 µg/mL in MCF-7 and MDA-MB-231 cells, respectively. The 24, 48, and 72 h treatments of 100 µg/mL SNWE showed 0.85 ± 0.07, 0.38 ± 0.1, and 0.20 ± 0.1 nM ATP in MCF-7 cells and 0.94 ± 0.07, 0.84 ± 0.2 and 0.46 ± 0.2 nM in MDA-MB-231 cells, respectively. The SNWE treatment altered the mitochondrial membrane potential (ΔΨm) in a concentration-dependent manner in both the breast cancer cell lines, to 29.6 ± 4.1% in MCF-7 and 28.7 ± 4.17% in MDA-MB-231 cells, when compared with healthy mitochondria (100% ΔΨm).<br />Conclusions: The cytotoxic effects of Solanum nigrum against breast cancer cells are associated with energy metabolism. Additional studies are warranted to test the anticancer effect of Solanum nigrum using an animal model of breast cancer.<br />Competing Interests: The authors declare no conflict of interest. HAK is serving as one of the Guest Editors and Editorial Board Members of this journal. We declare that HAK had no involvement in the peer review of this article and has no access to information regarding its peer review. Full responsibility for the editorial process for this article was delegated to SN.<br /> (© 2023 The Author(s). Published by IMR Press.)

Details

Language :
English
ISSN :
2768-6698
Volume :
28
Issue :
8
Database :
MEDLINE
Journal :
Frontiers in bioscience (Landmark edition)
Publication Type :
Academic Journal
Accession number :
37664945
Full Text :
https://doi.org/10.31083/j.fbl2808180