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CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2023 Sep 20; Vol. 71 (37), pp. 13577-13594. Date of Electronic Publication: 2023 Sep 01. - Publication Year :
- 2023
-
Abstract
- Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic acid targets can enable us to monitor food contamination in a timely manner. In recent years, the CRISPR/Cas system has been extensively explored in biosensing. However, there is a lack of a summary of CRISPR/Cas-powered detection tailored to non-nucleic acid targets involved in food safety. This review comprehensively summarizes the recent advances on the construction of CRISPR/Cas-powered detection and the promising applications in the field of food safety related non-nucleic acid targets. The current challenges and futuristic perspectives are also proposed accordingly. The rapidly evolving CRISPR/Cas system has provided a powerful propellant for non-nucleic acid target detection via integration with aptamer and/or DNAzyme. Compared with traditional analytical methods, CRISPR/Cas-powered detection is conceptually novel, essentially eliminates the dependence on large instruments, and also demonstrates the capability for rapid, accurate, sensitive, and on-site testing.
- Subjects :
- Food Safety
Food Contamination
Oligonucleotides
CRISPR-Cas Systems
DNA, Catalytic
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 71
- Issue :
- 37
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 37656446
- Full Text :
- https://doi.org/10.1021/acs.jafc.3c03619