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Highly Sensitive Cyanine Dyes for Rapid Sensing of NAD(P)H in Mitochondria and First-Instar Larvae of Drosophila melanogaster .
- Source :
-
ACS applied bio materials [ACS Appl Bio Mater] 2023 Aug 21; Vol. 6 (8), pp. 3199-3212. Date of Electronic Publication: 2023 Aug 09. - Publication Year :
- 2023
-
Abstract
- We have developed two highly sensitive cyanine dyes, which we refer to as probes A and B . These dyes are capable of quick and sensitive sensing of NAD(P)H. The dyes were fabricated by connecting benzothiazolium and 2,3-dimethylnaphtho[1,2- d ]thiazol-3-ium units to 3-quinolinium through a vinyl bond. In the absence of NAD(P)H, both probes have low fluorescence and absorption peaks at 370 and 400 nm, correspondingly. This is because of their two electron-withdrawing acceptor systems with high charge densities. However, when NAD(P)H reduces the probes' electron-withdrawing 3-quinolinium units to electron-donating 1,4-dihydroquinoline units, the probes absorb at 533 and 535 nm and fluoresce at 572 and 586 nm for A and B correspondingly. This creates well-defined donor-π-acceptor cyanine dyes. We successfully used probe A to monitor NAD(P)H levels in live cells during glycolysis, under hypoxic conditions induced by CoCl <subscript>2</subscript> treatment and after treatment with cancer drugs, including cisplatin, camptothecin, and gemcitabine. Probe A was also employed to visualize NAD(P)H in Drosophila melanogaster first-instar larvae. We observed an increase in NAD(P)H levels in A549 cancer cells both under hypoxic conditions and after treatment with cancer drugs, including cisplatin, camptothecin, and gemcitabine.
Details
- Language :
- English
- ISSN :
- 2576-6422
- Volume :
- 6
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- ACS applied bio materials
- Publication Type :
- Academic Journal
- Accession number :
- 37556116
- Full Text :
- https://doi.org/10.1021/acsabm.3c00320