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Role of ACC-deaminase synthesizing Trichoderma harzianum and plant growth-promoting bacteria in reducing salt-stress in Ocimum sanctum .

Authors :
Singh S
Chanotiya CS
Singh A
Vajpayee P
Kalra A
Source :
Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2023 Jun; Vol. 29 (6), pp. 815-828. Date of Electronic Publication: 2023 Jun 23.
Publication Year :
2023

Abstract

Salinity is a significant concern in crop production, causing severe losses in agricultural yields. Ocimum sanctum , also known as Holy Basil, is an important ancient medicinal plant used in the Indian traditional system of medicine. The present study explores the use of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing strains of plant-growth-promoting bacteria (PGPB) namely Str-8 ( Halomonas desiderata) , Sd-6 ( Brevibacterium halotolerans) , Fd-2 ( Achromobacter xylosoxidans ), Art-7 ( Burkholderia cepacia) , and Ldr-2 ( Bacillus subtilis) , and T. harzianum (Th), possessing multi-functional properties like growth promotion, stress alleviation, and for enhancing  O. sanctum  yield under salt stress. The results showed that co-inoculation of Th and PGPBs enhanced plant height and fresh herb weight by 3.78-17.65% and 7.86-58.76%, respectively; highest being in Th + Fd-2 and Th + Art-7 compared to positive control plants. The doubly inoculated plants showed increased pigments, phenol, flavonoids, protein, sugar, relative water content, and nutrient uptake (Nitrogen and Phosphorous) as compared to monocultures and untreated positive control plants. In addition, co-inoculation in plants resulted in lower Na <superscript>+</superscript> , MDA, H <subscript>2</subscript> O <subscript>2</subscript> , CAT, APX activities, and also lower ACC accumulation (49.75 to 72.38% compared to non-treated salt- stressed plant) in O. sanctum , which probably played a significant role in minimizing the deleterious effects of salinity. Finally, multifactorial analysis showed that co-inoculation of Th and PGPBs improved O. sanctum growth, its physiological activities, and alleviated salt stress compared to single inoculated and positive control plants. These microbial consortia were evaluated for the first time on  O. sanctum under salt stress. Therefore, the microbial consortia application could be employed to boost crop productivity in poor, marginalized and stressed agricultural fields.<br />Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01328-2.<br />Competing Interests: Conflict of interest The authors declare that they have no conflict of interest regarding the publication of this paper.<br /> (© Prof. H.S. Srivastava Foundation for Science and Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

Details

Language :
English
ISSN :
0971-5894
Volume :
29
Issue :
6
Database :
MEDLINE
Journal :
Physiology and molecular biology of plants : an international journal of functional plant biology
Publication Type :
Academic Journal
Accession number :
37520812
Full Text :
https://doi.org/10.1007/s12298-023-01328-2