On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies.

Chemokine receptors, a subfamily of G-protein-coupled receptors (GPCRs), are responsible for cell migration during physiological processes as well as in diseases like inflammation and cancers. Here, we present a protocol for solubilizing, purifying, and reconstituting complexes of chemokine receptors with their ligands in "nanodiscs," soluble lipid bilayers that mimic the native environment of membrane receptors. The protocol yields chemokine receptor complexes with sufficient purity and yield for structural and biophysical studies and should be applicable to other GPCRs.
Competing Interests: Declaration of interests Dr. Handel is a co-founder of Lassogen Inc and serves on the Scientific Advisory Boards of Artica, Abilita Bio, and Abalone Bio. The terms of these arrangements have been reviewed and approved by the University of California, San Diego, in accordance with its conflict of interest policies.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)