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α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2023 Sep; Vol. 299 (9), pp. 105091. Date of Electronic Publication: 2023 Jul 28. - Publication Year :
- 2023
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Abstract
- α-Synuclein and family members β- and γ-synuclein are presynaptic proteins that sense and generate membrane curvature, properties important for synaptic vesicle (SV) cycling. αβγ-synuclein triple knockout neurons exhibit SV endocytosis deficits. Here, we investigated if α-synuclein affects clathrin assembly in vitro. Visualizing clathrin assembly on membranes using a lipid monolayer system revealed that α-synuclein increases clathrin lattices size and curvature. On cell membranes, we observe that α-synuclein is colocalized with clathrin and its adapter AP180 in a concentric ring pattern. Clathrin puncta that contain both α-synuclein and AP180 were significantly larger than clathrin puncta containing either protein alone. We determined that this effect occurs in part through colocalization of α-synuclein with the phospholipid PI(4,5)P2 in the membrane. Immuno-electron microscopy (EM) of synaptosomes uncovered that α-synuclein relocalizes from SVs to the presynaptic membrane upon stimulation, positioning α-synuclein to function on presynaptic membranes during or after stimulation. Additionally, we show that deletion of synucleins impacts brain-derived clathrin-coated vesicle size. Thus, α-synuclein affects the size and curvature of clathrin structures on membranes and functions as an endocytic accessory protein.<br />Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.<br /> (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Cell Membrane metabolism
Endocytosis
Microscopy, Immunoelectron
Neurons metabolism
Presynaptic Terminals metabolism
Synaptosomes metabolism
Protein Transport
In Vitro Techniques
Phosphatidylinositol 4,5-Diphosphate metabolism
Brain cytology
Clathrin-Coated Vesicles metabolism
alpha-Synuclein genetics
alpha-Synuclein metabolism
Clathrin chemistry
Clathrin metabolism
Monomeric Clathrin Assembly Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 299
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 37516240
- Full Text :
- https://doi.org/10.1016/j.jbc.2023.105091