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Genetically Stable and Scalable Nanoengineering of Human Primary T Cells via Cell Mechanoporation.
- Source :
-
Nano letters [Nano Lett] 2023 Aug 23; Vol. 23 (16), pp. 7341-7349. Date of Electronic Publication: 2023 Jul 28. - Publication Year :
- 2023
-
Abstract
- Effective tumor regression has been observed with chimeric antigen receptor (CAR) T cells; however, the development of an affordable, safe, and effective CAR-T cell treatment remains a challenge. One of the major obstacles is that the suboptimal genetic modification of T cells reduces their yield and antitumor activity, necessitating the development of a next-generation T cell engineering approach. In this study, we developed a nonviral T cell nanoengineering system that allows highly efficient delivery of diverse functional nanomaterials into primary human T cells in a genetically stable and scalable manner. Our platform leverages the unique cell deformation and restoration process induced by the intrinsic inertial flow in a microchannel to create nanopores in the cellular membrane for macromolecule internalization, leading to effective transfection with high scalability and viability. The proposed approach demonstrates considerable potential as a practical alternative technique for improving the current CAR-T cell manufacturing process.
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 23
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 37506062
- Full Text :
- https://doi.org/10.1021/acs.nanolett.3c01720