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Immunohistochemical expression of HMGB1 and related proteins in the skin as a possible tool for determining post-mortem interval: a preclinical study.
- Source :
-
Forensic science, medicine, and pathology [Forensic Sci Med Pathol] 2024 Mar; Vol. 20 (1), pp. 149-165. Date of Electronic Publication: 2023 Jul 25. - Publication Year :
- 2024
-
Abstract
- Determining the post-mortem interval (PMI) is one of forensic pathology's primary objectives and one of its most challenging tasks. Numerous studies have demonstrated the accuracy of histomorphology and immunohistochemical investigations in determining the time of death. Nevertheless, the skin, a robust and easy-to-remove tissue, has only been partially analyzed so far. By studying 20 adult male mice, we tried to determine whether post-mortem immunohistochemical detection in the skin of HMGB1 proteins and associated components (Beclin1 and RAGE) could be used for this purpose. We discovered that nuclear HMGB1 overexpression indicates that death occurred within the previous 12 h, nuclear HMGB1 negativization with high cytoplasmic HMGB1 intensity indicates that death occurred between 12 and 36 h earlier and cytoplasmic HMGB1 negativization indicates that more than 48 h have passed since death. RAGE and Beclin1 levels in the cytoplasm also decreased with time. The latter proteins' negativization might indicate that more than 24 and 36 h, respectively, have passed from the time of death. These indicators might potentially be helpful in forensic practice for determining the PMI using immunohistochemistry.<br /> (© 2023. The Author(s).)
- Subjects :
- Male
Mice
Animals
Beclin-1
Autopsy
Time
Postmortem Changes
HMGB1 Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1556-2891
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Forensic science, medicine, and pathology
- Publication Type :
- Academic Journal
- Accession number :
- 37490201
- Full Text :
- https://doi.org/10.1007/s12024-023-00634-1