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Investigating the aggregation perspective of Dengue virus proteome.
- Source :
-
Virology [Virology] 2023 Sep; Vol. 586, pp. 12-22. Date of Electronic Publication: 2023 Jul 13. - Publication Year :
- 2023
-
Abstract
- Dengue viruses are human pathogens that are transmitted through mosquitoes. Apart from the typical symptoms associated with viral fevers, DENV infections are known to cause several neurological complications such as meningitis, encephalitis, intracranial haemorrhage, retinopathies along with the more severe, and sometimes fatal, vascular leakage and dengue shock syndrome. This study was designed to investigate, in detail, the predicted viral protein aggregation prone regions among all serotypes. Further, in order to understand the cross-talk between viral protein aggregation and aggregation of cellular proteins, cross-seeding experiments between the DENV NS1 (1-30), corresponding to the β-roll domain and the diabetes hallmark protein, amylin, were performed. Various techniques such as fluorescence spectroscopy, circular dichroism, atomic force microscopy and immunoblotting have been employed for this. We observe that the DENV proteomes have many predicted APRs and the NS1 (1-30) of DENV1-3, 2K and capsid anchor of DENV2 and DENV4 are capable of forming amyloids, in vitro. Further, the DENV NS1 (1-30), aggregates are also able to cross-seed and enhance amylin aggregation and vice-versa. This knowledge may lead to an opportunity for designing suitable inhibitors of protein aggregation that may be beneficial for viral infections and comorbidities.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Subjects :
- Proteome
Islet Amyloid Polypeptide metabolism
Protein Aggregates
Humans
Dengue metabolism
Dengue pathology
Dengue virology
Diabetes Mellitus, Type 2 metabolism
Diabetes Mellitus, Type 2 pathology
Dengue Virus chemistry
Dengue Virus classification
Viral Proteins chemistry
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 586
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 37473502
- Full Text :
- https://doi.org/10.1016/j.virol.2023.07.010