BSA-assisted ultrasound synthesis of water stable CsPbBr 3 nanocrystals for sensitive fluorescent detection of hydrogen sulfide in human serum.

A bovine serum albumin (BSA)-assisted ultrasonication strategy was developed for the synthesis of CsPbBr ₃ nanocrystals (NCs) with stable fluorescence properties in aqueous solution. Such a preparation method is simple, fast and does not require complex equipment. The results show that the synthesized CsPbBr ₃ NCs are homogeneous in particle size and have good solubility and stability in water. The CsPbBr ₃ NCs have been utilized as fluorescence probe for rapid detection of hydrogen sulfide (H ₂ S) in human serum. The reaction of H ₂ S with the lead sites on the surface of CsPbBr ₃ NCs produces lead sulfide (PbS), resulting in the decrease of fluorometric intensity of CsPbBr ₃ NCs. Our designed fluorescent assay has a linear S ^2- detecting range of 10 ~ 800 nM with a detection limit of 7.05 nM. The assay was used to determine H ₂ S in human serum with spiked recoveries ranging from 94.98% to 102.69%. This work opens new avenues for the application of halide lead perovskite in different biosensing areas.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)