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Shedding light on human olfaction: Electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium.

Authors :
Hernandez-Clavijo A
Sánchez Triviño CA
Guarneri G
Ricci C
Mantilla-Esparza FA
Gonzalez-Velandia KY
Boscolo-Rizzo P
Tofanelli M
Bonini P
Dibattista M
Tirelli G
Menini A
Source :
IScience [iScience] 2023 Jun 21; Vol. 26 (7), pp. 107186. Date of Electronic Publication: 2023 Jun 21 (Print Publication: 2023).
Publication Year :
2023

Abstract

The COVID-19 pandemic brought attention to our limited understanding of human olfactory physiology. While the cellular composition of the human olfactory epithelium is similar to that of other vertebrates, its functional properties are largely unknown. We prepared acute slices of human olfactory epithelium from nasal biopsies and used the whole-cell patch-clamp technique to record electrical properties of cells. We measured voltage-gated currents in human olfactory sensory neurons and supporting cells, and action potentials in neurons. Additionally, neuronal inward current and action potentials responses to a phosphodiesterase inhibitor suggested a transduction cascade involving cAMP as a second messenger. Furthermore, responses to odorant mixtures demonstrated that the transduction cascade was intact in this preparation. This study provides the first electrophysiological characterization of olfactory sensory neurons in acute slices of the human olfactory epithelium, paving the way for future research to expand our knowledge of human olfactory physiology.<br />Competing Interests: The authors declare no competing financial interests.<br /> (© 2023 The Author(s).)

Details

Language :
English
ISSN :
2589-0042
Volume :
26
Issue :
7
Database :
MEDLINE
Journal :
IScience
Publication Type :
Academic Journal
Accession number :
37456832
Full Text :
https://doi.org/10.1016/j.isci.2023.107186