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Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2690, pp. 23-36. - Publication Year :
- 2023
-
Abstract
- The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting protein partners. With the introduction of mating-based methods for split-ubiquitin screens, the approach has gained broad popularity because of its exceptionally high transformation efficiency, utility in working with full-length membrane proteins, and positive selection with little interference from spurious interactions. Recent advances now extend these split-ubiquitin methods to the analysis of interactions between otherwise soluble proteins and tripartite protein interactions.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2690
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 37450134
- Full Text :
- https://doi.org/10.1007/978-1-0716-3327-4_3