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Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine.
- Source :
-
Cell reports [Cell Rep] 2023 Jul 25; Vol. 42 (7), pp. 112780. Date of Electronic Publication: 2023 Jul 12. - Publication Year :
- 2023
-
Abstract
- Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike <superscript>+</superscript> (S-2P <superscript>+</superscript> ) and S-2P <superscript>-</superscript> B cells reveal clonal expansion and accumulating mutations among S-2P <superscript>+</superscript> cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3 <superscript>+</superscript> MBCs. One branch leads to CD11c <superscript>+</superscript> atypical MBCs while the other develops from CD71 <superscript>+</superscript> activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P <superscript>+</superscript> clones, several are populated with plasmablasts at early timepoints as well as CD71 <superscript>+</superscript> activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs.<br />Competing Interests: Declaration of interests S.H.K. receives consulting fees from Peraton. K.B.H. receives consulting fees from Prellis Biologics.<br /> (Published by Elsevier Inc.)
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 42
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 37440409
- Full Text :
- https://doi.org/10.1016/j.celrep.2023.112780