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Generation of an Avian Myeloblastosis Virus (AMV) Reverse Transcriptase Prime Editor.
- Source :
-
Advances in experimental medicine and biology [Adv Exp Med Biol] 2023; Vol. 1415, pp. 109-114. - Publication Year :
- 2023
-
Abstract
- Prime editing (PE) is a novel, double-strand break (DSB)-independent gene editing technology that represents an exciting avenue for the treatment of inherited retinal diseases (IRDs). Given the extensive and heterogenous nature of the 280 genes associated with IRDs, genome editing has presented countless complications. However, recent advances in genome editing technologies have identified PE to have tremendous potential, with the capability to ameliorate small deletions and insertions in addition to all twelve possible transition and transversion mutations. The current PE system is based on the fusion of the Streptococcus pyogenes Cas9 (SpCas9) nickase H840A mutant and an optimized Moloney murine leukemia virus (MMLV) reverse-transcriptase (RT) in conjunction with a PE guide RNA (pegRNA). In this study, we developed a prime editor based on the avian myeloblastosis virus (AMV)-RT and showed its applicability for the installation of the PRPH2 c.828+1G>A mutation in HEK293 cells.<br /> (© 2023. The Author(s), under exclusive license to Springer Nature Switzerland AG.)
- Subjects :
- Humans
Animals
Mice
HEK293 Cells
Gene Editing
Moloney murine leukemia virus genetics
Moloney murine leukemia virus metabolism
CRISPR-Cas Systems
RNA-Directed DNA Polymerase genetics
RNA-Directed DNA Polymerase metabolism
Avian Myeloblastosis Virus genetics
Avian Myeloblastosis Virus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0065-2598
- Volume :
- 1415
- Database :
- MEDLINE
- Journal :
- Advances in experimental medicine and biology
- Publication Type :
- Academic Journal
- Accession number :
- 37440022
- Full Text :
- https://doi.org/10.1007/978-3-031-27681-1_17