Responses of CH 4 , N 2 O, and NH 3 emissions to different slurry pH values of 5.5-10.0: Characteristics and mechanisms.

Animal slurry storage is a significant source of greenhouse gas (GHG) and ammonia (NH ₃ ) emissions. pH is a basic but key factor that could pose great influence on gas emissions, but the simultaneous evaluation of its influence on GHG and NH ₃ emissions and the understanding of its underlying mechanism are not enough. In this work, pH was adjusted between 5.5 and 10.0 by a step of 0.5 unit by adding lactic acid and sodium hydroxide (NaOH) properly and frequently to the stored slurry during a 43-day storage period. The cumulative NH ₃ emissions were linearly correlated with the slurry pH, with R ² being 0.982. Maintaining the slurry pH at 5.5-6.0 could reduce NH ₃ emissions by 69.4%-85.1% compared with the non-treated group (CK). The pH ranges for maximum methane (CH ₄ ) and nitrous oxide (N ₂ O) emissions were 7.5-8.5 and 6.5-8.5, respectively, and the slurry under pH 7.5-8.5 showed the highest GHG emissions. Acidification to pH 5.5 helped reduce the CH ₄ , N ₂ O, and total GHG emissions by 98.0%, 29.3%, and 81.7%, respectively; while alkalinization to pH 10.0 helped achieve the mitigation effects of 74.1%, 24.9%, and 30.6%, respectively. The Pearson's correlation factor between CH ₄ and the gene copy of mcrA under different pH values was 0.744 (p < 0.05). Meanwhile, the correlation factors between N ₂ O and the gene copies of amoA, narG, and nirS were 0.644 (p < 0.05), 0.719 (p < 0.05), and 0.576 (p = 0.081), respectively. The gene copies of mcrA, amoA, narG, and nirS were maintained at the lowest level under pH 5.5. These results recommended keeping slurry pH lower than 5.5 with lactic acid can help control GHG and NH ₃ emissions simultaneously and effectively.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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