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Insulin and glucagon secretion from rat islets maintained in a tissue culture-perifusion system.
- Source :
-
Diabetes [Diabetes] 1979 Apr; Vol. 28 (4), pp. 276-81. - Publication Year :
- 1979
-
Abstract
- A tissue culture-perifusion system is described that allows for long-term culture of pancreatic islets and study of the dynamics of islet hormone secretion. Islets cultured in this system demonstrate brisk, reproducible biphasic insulin and glucagon release. Glucose-stimulated insulin release is similar after 1 or 14 days in culture. Freshly isolated islets are relatively insensitive to somatostatin, requiring 100 ng/ml to suppress partially the glucose-induced insulin secretion. After 24 h of culture, the same islets demonstrate a marked increase in sensitivity to this hormone. Glucagon secretion from islets maintained in this system occurred in a predictable fashion to arginine stimulation and glucose inhibition.
Details
- Language :
- English
- ISSN :
- 0012-1797
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Diabetes
- Publication Type :
- Academic Journal
- Accession number :
- 374172
- Full Text :
- https://doi.org/10.2337/diab.28.4.276