Trait matching in a multi-species geographic mosaic of leafflower plants, brood pollinators, and cheaters.

Trait matching between mutualistic species is usually expected to maintain mutualism, but empirical studies of trait complementarity and coadaptation in multi-species assemblages-which represent the reality of most interactions in nature-are few. Here, we studied trait matching between the leafflower shrub Kirganelia microcarpa and three associated seed-predatory leafflower moths ( Epicephala spp.) across 16 populations. Behavioral and morphological observations suggested that two moths ( E. microcarpa and E. tertiaria ) acted as pollinators while a third ( E. laeviclada ) acted as a cheater. These species differed in ovipositor morphology but showed trait complementarity between ovipositor length and floral traits at both species level and population level, presumably as adaptations to divergent oviposition behaviors. However, this trait matching varied among populations. Comparisons of ovipositor length and floral traits among populations with different moth assemblages suggested an increase of ovary wall thickness where the locular-ovipositing pollinator E. microcarpa and cheater E. laeviclada were present, while stylar pit depth was less in populations with the stylar pit-ovipositing pollinator E. tertiaria . Our study indicates that trait matching between interacting partners occurs even in extremely specialized multi-species mutualisms, and that although these responses vary, sometimes non-intuitively, in response to different partner species. It seems that the moths can track changes in host plant tissue depth for oviposition.
Competing Interests: The authors declare that they have no conflict of interest.
(© 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)