Back to Search Start Over

Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirus-Insect Cell Expression System.

Authors :
Sivaraman H
Wong W
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2691, pp. 199-206.
Publication Year :
2023

Abstract

Inflammasomes are innate immune sensing and signaling complexes critical for defense against pathogens and response to cellular stresses. A core component of inflammasomes is the sensor protein, which, upon sensing pathogen- or danger-associated molecular patterns (PAMPs or DAMPs), converts from inactive to active signaling platform for initiation of inflammatory signaling. A reliable source for the production and purification of recombinant inflammasome sensors is therefore invaluable for biochemical and structural characterizations, as well as drug screening for the development of therapeutics. Here, we describe an expression and purification protocol using the baculovirus-insect cell expression system to generate recombinant NLRP1, an important member of the NOD-like receptor (NLR) family of inflammasome sensors.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2691
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
37355547
Full Text :
https://doi.org/10.1007/978-1-0716-3331-1_15