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Shedding light on the electron transfer chain of a moderately acidophilic iron oxidizer: characterization of recombinant HiPIP-41, CytC-18 and CytC-78 derived from Ferrovum sp. PN-J47-F6.

Authors :
Ullrich SR
Fuchs H
Schlömann M
Source :
Research in microbiology [Res Microbiol] 2024 Jan-Feb; Vol. 175 (1-2), pp. 104088. Date of Electronic Publication: 2023 Jun 20.
Publication Year :
2024

Abstract

Efficient electron transfer from the donor to the acceptor couple presents a necessary requirement for acidophilic and neutrophilic iron oxidizers due to the low energy yield of aerobic ferrous iron oxidation. Involved periplasmic electron carriers are very diverse in these bacteria and show adaptations to the respective thermodynamic constraints such as a more positive redox potential reported for extreme acidophilic Acidithiobacillus spp. Respiratory chain candidates of moderately acidophilic members of the genus Ferrovum share similarities with both their neutrophilic iron oxidizing relatives and the more distantly related Acidithiobacillus spp. We examined our previous omics-based conclusions on the potential electron transfer chain in Ferrovum spp. by characterizing the three redox protein candidates CytC-18, CytC-78 and HiPIP-41 of strain PN-J47-F6 which were produced as recombinant proteins in Eschericha coli. UV/Vis-based redox assays suggested that HiPIP-41 has a very positive redox potential while redox potentials of CytC-18 and CytC-78 are more negative than their counterparts in Acidithiobacillus spp. Far Western dot blotting demonstrated interactions between all three recombinant redox proteins while redox assays showed the electron transfer from HiPIP-41 to either of the cytochromes. Altogether, CytC-18, CytC-78 and HiPIP-41 indeed represent very likely candidates of the electron transfer in Ferrovum sp. PN-J4-F6.<br /> (Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)

Details

Language :
English
ISSN :
1769-7123
Volume :
175
Issue :
1-2
Database :
MEDLINE
Journal :
Research in microbiology
Publication Type :
Academic Journal
Accession number :
37348744
Full Text :
https://doi.org/10.1016/j.resmic.2023.104088